Differential expression of integrins and laminin-5 in normal oral epithelia

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Standard

Differential expression of integrins and laminin-5 in normal oral epithelia. / Thorup, A K; Dabelsteen, Erik; Schou, S; Gil, S G; Carter, W G; Reibel, J.

In: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 105, No. 7, 07.1997, p. 519-30.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Thorup, AK, Dabelsteen, E, Schou, S, Gil, SG, Carter, WG & Reibel, J 1997, 'Differential expression of integrins and laminin-5 in normal oral epithelia', APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, vol. 105, no. 7, pp. 519-30.

APA

Thorup, A. K., Dabelsteen, E., Schou, S., Gil, S. G., Carter, W. G., & Reibel, J. (1997). Differential expression of integrins and laminin-5 in normal oral epithelia. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 105(7), 519-30.

Vancouver

Thorup AK, Dabelsteen E, Schou S, Gil SG, Carter WG, Reibel J. Differential expression of integrins and laminin-5 in normal oral epithelia. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 1997 Jul;105(7):519-30.

Author

Thorup, A K ; Dabelsteen, Erik ; Schou, S ; Gil, S G ; Carter, W G ; Reibel, J. / Differential expression of integrins and laminin-5 in normal oral epithelia. In: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 1997 ; Vol. 105, No. 7. pp. 519-30.

Bibtex

@article{7d5bff23ee88472481433fa255eddd4a,
title = "Differential expression of integrins and laminin-5 in normal oral epithelia",
abstract = "beta 1 and beta 4 integrins are receptors on epithelial cells mediating cell-extracellular matrix adhesion. Furthermore, alpha 2 beta 1 and alpha 3 beta 1 contribute to cell-cell adhesion. Laminin-5 in epithelial basement membranes (BMs) is a ligand for alpha 6 beta 4 and alpha 3 beta 1. Expression of different integrins and laminin-5 was studied in oral epithelium to characterize regional variations in these adhesion molecules. Monoclonal antibodies directed against alpha 2-alpha 6 beta 1/alpha 6 beta 4 and laminin-5 were examined in cryopreserved biopsies of normal mucosa by immunohistochemistry. Laminin-5 was expressed as a line along the BMs. The junctional epithelium showed a unique phenotype: Laminin-5 was detected in the internal BM at the tooth surface and in the external BM, where excessive laminin-5 was seen in the stroma. alpha 6 beta 4 was expressed in all cells of the junctional epithelium. Integrins alpha 4 beta 1 and alpha 5 beta 1 were not detected in the epithelia, whereas alpha 2 beta 1 and alpha 3 beta 1 showed differential expression. Epithelia with well-developed rete pegs and connective tissue papillae showed polarized alpha 3 beta 1 expression along the BM in the rete pegs, in contrast to negative expression at the tips of the connective tissue papillae. A variation in the suprabasal distribution of alpha 2 beta 1 and alpha 3 beta 1 was observed between epithelia from different regions. alpha 2 beta 1 and alpha 3 beta 1 were detected in basal/parabasal cells in keratinized epithelia, whereas there was increased suprabasal expression in nonkeratinized mucosa. These results indicate inhomogeneity in the basal cell population of oral squamous epithelia and differential expression of integrins, which may reflect differences in the underlying stroma. Laminin-5 deposits in the stroma underneath the junctional epithelium may indicate subclinical gingival inflammation.",
keywords = "Adult, Animals, Basement Membrane, Cell Adhesion Molecules, Epithelium, Female, Humans, Integrin alpha3beta1, Integrins, Male, Mice, Middle Aged, Mouth Mucosa, Rats, Receptors, Collagen, Receptors, Fibronectin, Tissue Distribution",
author = "Thorup, {A K} and Erik Dabelsteen and S Schou and Gil, {S G} and Carter, {W G} and J Reibel",
year = "1997",
month = jul,
language = "English",
volume = "105",
pages = "519--30",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",
number = "7",

}

RIS

TY - JOUR

T1 - Differential expression of integrins and laminin-5 in normal oral epithelia

AU - Thorup, A K

AU - Dabelsteen, Erik

AU - Schou, S

AU - Gil, S G

AU - Carter, W G

AU - Reibel, J

PY - 1997/7

Y1 - 1997/7

N2 - beta 1 and beta 4 integrins are receptors on epithelial cells mediating cell-extracellular matrix adhesion. Furthermore, alpha 2 beta 1 and alpha 3 beta 1 contribute to cell-cell adhesion. Laminin-5 in epithelial basement membranes (BMs) is a ligand for alpha 6 beta 4 and alpha 3 beta 1. Expression of different integrins and laminin-5 was studied in oral epithelium to characterize regional variations in these adhesion molecules. Monoclonal antibodies directed against alpha 2-alpha 6 beta 1/alpha 6 beta 4 and laminin-5 were examined in cryopreserved biopsies of normal mucosa by immunohistochemistry. Laminin-5 was expressed as a line along the BMs. The junctional epithelium showed a unique phenotype: Laminin-5 was detected in the internal BM at the tooth surface and in the external BM, where excessive laminin-5 was seen in the stroma. alpha 6 beta 4 was expressed in all cells of the junctional epithelium. Integrins alpha 4 beta 1 and alpha 5 beta 1 were not detected in the epithelia, whereas alpha 2 beta 1 and alpha 3 beta 1 showed differential expression. Epithelia with well-developed rete pegs and connective tissue papillae showed polarized alpha 3 beta 1 expression along the BM in the rete pegs, in contrast to negative expression at the tips of the connective tissue papillae. A variation in the suprabasal distribution of alpha 2 beta 1 and alpha 3 beta 1 was observed between epithelia from different regions. alpha 2 beta 1 and alpha 3 beta 1 were detected in basal/parabasal cells in keratinized epithelia, whereas there was increased suprabasal expression in nonkeratinized mucosa. These results indicate inhomogeneity in the basal cell population of oral squamous epithelia and differential expression of integrins, which may reflect differences in the underlying stroma. Laminin-5 deposits in the stroma underneath the junctional epithelium may indicate subclinical gingival inflammation.

AB - beta 1 and beta 4 integrins are receptors on epithelial cells mediating cell-extracellular matrix adhesion. Furthermore, alpha 2 beta 1 and alpha 3 beta 1 contribute to cell-cell adhesion. Laminin-5 in epithelial basement membranes (BMs) is a ligand for alpha 6 beta 4 and alpha 3 beta 1. Expression of different integrins and laminin-5 was studied in oral epithelium to characterize regional variations in these adhesion molecules. Monoclonal antibodies directed against alpha 2-alpha 6 beta 1/alpha 6 beta 4 and laminin-5 were examined in cryopreserved biopsies of normal mucosa by immunohistochemistry. Laminin-5 was expressed as a line along the BMs. The junctional epithelium showed a unique phenotype: Laminin-5 was detected in the internal BM at the tooth surface and in the external BM, where excessive laminin-5 was seen in the stroma. alpha 6 beta 4 was expressed in all cells of the junctional epithelium. Integrins alpha 4 beta 1 and alpha 5 beta 1 were not detected in the epithelia, whereas alpha 2 beta 1 and alpha 3 beta 1 showed differential expression. Epithelia with well-developed rete pegs and connective tissue papillae showed polarized alpha 3 beta 1 expression along the BM in the rete pegs, in contrast to negative expression at the tips of the connective tissue papillae. A variation in the suprabasal distribution of alpha 2 beta 1 and alpha 3 beta 1 was observed between epithelia from different regions. alpha 2 beta 1 and alpha 3 beta 1 were detected in basal/parabasal cells in keratinized epithelia, whereas there was increased suprabasal expression in nonkeratinized mucosa. These results indicate inhomogeneity in the basal cell population of oral squamous epithelia and differential expression of integrins, which may reflect differences in the underlying stroma. Laminin-5 deposits in the stroma underneath the junctional epithelium may indicate subclinical gingival inflammation.

KW - Adult

KW - Animals

KW - Basement Membrane

KW - Cell Adhesion Molecules

KW - Epithelium

KW - Female

KW - Humans

KW - Integrin alpha3beta1

KW - Integrins

KW - Male

KW - Mice

KW - Middle Aged

KW - Mouth Mucosa

KW - Rats

KW - Receptors, Collagen

KW - Receptors, Fibronectin

KW - Tissue Distribution

M3 - Journal article

C2 - 9269298

VL - 105

SP - 519

EP - 530

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 7

ER -

ID: 119594091