Gluten-free diet modulates inflammation in salivary glands and pancreatic islets
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Gluten-free diet modulates inflammation in salivary glands and pancreatic islets. / Haupt-Jorgensen, Martin; Groule, Vibeke; Reibel, Jesper; Buschard, Karsten; Pedersen, Anne Marie Lynge.
In: Oral Diseases, Vol. 28, No. 3, 28, 2022, p. 639-647.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Gluten-free diet modulates inflammation in salivary glands and pancreatic islets
AU - Haupt-Jorgensen, Martin
AU - Groule, Vibeke
AU - Reibel, Jesper
AU - Buschard, Karsten
AU - Pedersen, Anne Marie Lynge
N1 - This article is protected by copyright. All rights reserved.
PY - 2022
Y1 - 2022
N2 - OBJECTIVES: A lifelong gluten-free (GF) diet ameliorates autoimmune diabetes in non-obese diabetic (NOD) mice and most likely in humans. Besides diabetes, NOD mice develop focal sialadenitis, as seen in Sjögren's syndrome (SS). In humans, type 1 diabetes (T1D) is also linked to SS. Here we investigated whether a lifelong GF diet influences the immune cell infiltration in the salivary glands and pancreatic islets in NOD mice.METHODS: NOD mice were fed a lifelong (i.e. 13 weeks) GF or gluten-containing standard (STD) diet. Insulitis and sialadenitis were scored on H&E-stained paraffin-embedded sections of pancreas and submandibular glands. Immune-cell specificity and distribution were investigated immunohistochemically.RESULTS: There were fewer CD68+ and CD4+ cells in submandibular gland areas with focal sialadenitis as well as reduced insulitis and fewer VEGFR2+ cells in pancreatic islets in mice on GF versus STD diet. The degree of sialadenitis was not significantly lower in GF mice, but sialadenitis and insulitis correlated strongly. Lung weight was lower in GF mice.CONCLUSION: In NOD mice, a lifelong GF diet reduces infiltration of monocytes/macrophages and T cells in salivary glands and inflammation in pancreatic islets, possibly by reducing VEGFR2, indicating that the linked autoimmune diseases, T1D and SS, may be alleviated by a GF diet.
AB - OBJECTIVES: A lifelong gluten-free (GF) diet ameliorates autoimmune diabetes in non-obese diabetic (NOD) mice and most likely in humans. Besides diabetes, NOD mice develop focal sialadenitis, as seen in Sjögren's syndrome (SS). In humans, type 1 diabetes (T1D) is also linked to SS. Here we investigated whether a lifelong GF diet influences the immune cell infiltration in the salivary glands and pancreatic islets in NOD mice.METHODS: NOD mice were fed a lifelong (i.e. 13 weeks) GF or gluten-containing standard (STD) diet. Insulitis and sialadenitis were scored on H&E-stained paraffin-embedded sections of pancreas and submandibular glands. Immune-cell specificity and distribution were investigated immunohistochemically.RESULTS: There were fewer CD68+ and CD4+ cells in submandibular gland areas with focal sialadenitis as well as reduced insulitis and fewer VEGFR2+ cells in pancreatic islets in mice on GF versus STD diet. The degree of sialadenitis was not significantly lower in GF mice, but sialadenitis and insulitis correlated strongly. Lung weight was lower in GF mice.CONCLUSION: In NOD mice, a lifelong GF diet reduces infiltration of monocytes/macrophages and T cells in salivary glands and inflammation in pancreatic islets, possibly by reducing VEGFR2, indicating that the linked autoimmune diseases, T1D and SS, may be alleviated by a GF diet.
U2 - 10.1111/odi.13775
DO - 10.1111/odi.13775
M3 - Journal article
C2 - 33432638
VL - 28
SP - 639
EP - 647
JO - Oral Diseases
JF - Oral Diseases
SN - 1354-523X
IS - 3
M1 - 28
ER -
ID: 255405713