Non-specific esterases in partly mineralized bovine enamel
Research output: Contribution to journal › Journal article › Research › peer-review
Activity for non-specific esterase was demonstrated in the matrix of developing bovine enamel with alpha-naphthyl acetate and 5-bromoindoxyl acetate as the esterase substrates. By use of high-performance liquid chromatography gel filtration, ion-exchange chromatography, and electrophoresis three esterases were shown to be present in the enamel matrix. The enzymes showed highest activity at pH 6.5-7.5. In sections a strong reaction was observed in the secretory ameloblasts. The esterases may be proteolytic enzymes that participate in the degradation of the matrix proteins.
Original language | English |
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Journal | Acta Odontologica Scandinavica |
Volume | 48 |
Issue number | 5 |
Pages (from-to) | 327-32 |
Number of pages | 5 |
ISSN | 0001-6357 |
Publication status | Published - 1990 |
Bibliographical note
Keywords: Amelogenesis; Animals; Cattle; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Dental Enamel; Dental Enamel Proteins; Electrophoresis, Polyacrylamide Gel; Esterases; Indoles; Naphthol AS D Esterase; Spectrophotometry; Tooth Germ
ID: 10140780