Single cell migration in oral squamous cell carcinoma - possible evidence of epithelial-mesenchymal transition in vivo
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Single cell migration in oral squamous cell carcinoma - possible evidence of epithelial-mesenchymal transition in vivo. / Jensen, David H; Reibel, Jesper; Mackenzie, Ian C; Dabelsteen, Erik.
In: Journal of Oral Pathology & Medicine, Vol. 44, No. 9, 10.2015, p. 674-679.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Single cell migration in oral squamous cell carcinoma - possible evidence of epithelial-mesenchymal transition in vivo
AU - Jensen, David H
AU - Reibel, Jesper
AU - Mackenzie, Ian C
AU - Dabelsteen, Erik
N1 - © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
PY - 2015/10
Y1 - 2015/10
N2 - BACKGROUND: The invasion of cancer cells into the surrounding normal tissue is one of the defining features of cancer. While the phenomena of tumour budding, epithelial-mesenchymal transition and the presence of myofibroblasts have independently been shown to be related to a poor prognosis of oral carcinomas, their relationship has not been examined in detail.METHODS: Paraffin-embedded tissues from 28 patients with oral squamous cell carcinomas were stained with antibodies to cytokeratin, α-SMA, vimentin, E-cadherin, N-cadherin and Twist and evaluated for their expression in relation to invasive cancer cells and the surrounding tumour stroma.RESULTS AND CONCLUSIONS: A direct, histological relationship between invading, budding tumour cells and myofibroblasts was occasionally seen but was not a general feature. Most of the budding tumour cells at the invasive front had a decreased expression of E-cadherin, but we did not find that this was associated with a consistent or clear increase in either N-cadherin or vimentin. We therefore suggest that the budding of tumour cells is not dependent upon either myofibroblasts or a complete epithelial-mesenchymal transition and that these phenomena most likely represent separate processes in tumour progression.
AB - BACKGROUND: The invasion of cancer cells into the surrounding normal tissue is one of the defining features of cancer. While the phenomena of tumour budding, epithelial-mesenchymal transition and the presence of myofibroblasts have independently been shown to be related to a poor prognosis of oral carcinomas, their relationship has not been examined in detail.METHODS: Paraffin-embedded tissues from 28 patients with oral squamous cell carcinomas were stained with antibodies to cytokeratin, α-SMA, vimentin, E-cadherin, N-cadherin and Twist and evaluated for their expression in relation to invasive cancer cells and the surrounding tumour stroma.RESULTS AND CONCLUSIONS: A direct, histological relationship between invading, budding tumour cells and myofibroblasts was occasionally seen but was not a general feature. Most of the budding tumour cells at the invasive front had a decreased expression of E-cadherin, but we did not find that this was associated with a consistent or clear increase in either N-cadherin or vimentin. We therefore suggest that the budding of tumour cells is not dependent upon either myofibroblasts or a complete epithelial-mesenchymal transition and that these phenomena most likely represent separate processes in tumour progression.
U2 - 10.1111/jop.12321
DO - 10.1111/jop.12321
M3 - Journal article
C2 - 25880532
VL - 44
SP - 674
EP - 679
JO - Journal of Oral Pathology and Medicine
JF - Journal of Oral Pathology and Medicine
SN - 0904-2512
IS - 9
ER -
ID: 135643491