Chromatographic separation of alkaline phosphatase from dental enamel
Research output: Contribution to journal › Journal article › Research › peer-review
Alkaline phosphatase (AP) was prepared from partly mineralized bovine enamel by extraction in phosphate buffer, centrifugation and various chromatographic techniques. Chromatofocusing showed that the enamel enzyme possessed five isoelectric points at the acid pH level ranging from pH 5.7 to pH 4.4. Three enzyme peaks were eluted using low pressure chromatography with a Bio-gel column. With a HPLC gel filtration column the separation of the enamel extract resulted in only one peak with AP activity. The fractions of this peak were used to produce an antibody against bovine AP.
Original language | English |
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Journal | Scandinavian Journal of Dental Research |
Volume | 97 |
Issue number | 1 |
Pages (from-to) | 8-13 |
Number of pages | 5 |
ISSN | 0029-845X |
Publication status | Published - 1989 |
Bibliographical note
Keywords: Alkaline Phosphatase; Animals; Cattle; Chromatography; Chromatography, Gel; Chromatography, High Pressure Liquid; Dental Enamel; Dental Enamel Proteins; Electrophoresis; Isoelectric Focusing; Spectrophotometry
ID: 10141289