Comparison of two chair-side tests for enumeration of Mutans Streptococci in saliva
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Comparison of two chair-side tests for enumeration of Mutans Streptococci in saliva. / Twetman, Lisa; Twetman, Svante.
In: Oral health and dental management, Vol. 13, No. 3, 630, 09.2014, p. 580-3.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Comparison of two chair-side tests for enumeration of Mutans Streptococci in saliva
AU - Twetman, Lisa
AU - Twetman, Svante
PY - 2014/9
Y1 - 2014/9
N2 - AIM: To compare the prevalence and levels of salivary Mutans Streptococci (MS) assessed with two commercial chair-side methods based on culture growth or monoclonal antibodies, respectively.MATERIAL AND METHODS: The study group consisted of a convenience sample of 89 adults, 23-72 years of age, referred to a maxillofacial hospital clinic with a caries history. Stimulated whole saliva samples were collected and the number of MS was assessed with the Dentocult-SM Strip Mutans (DSM) and the Saliva-Check Mutans (SCM). The outcome was compared with conventional anaerobic laboratory cultivation on selective MSB agar.RESULTS: The prevalence of ≥ 5 × 10(5) CFU was 21%, 62%, and 73% with the SCM, DSM and MSB-cultivation, respectively. The correlation between DSM and SCM tests was r = 0.49 (p < 0.05) but the agreement on the high levels was fair. In comparison with the MSB agar, DSM provided acceptable levels of sensitivity and specificity while the SCM displayed a poor sensitivity but a perfect specificity. Both chair-side methods were significantly related to the prevalence of active root caries lesions (p < 0.05).CONCLUSIONS: The two chair-side salivary tests were significantly correlated and due their high specificity, they may be sensitive enough for screening purposes and for patient-centered promotion of oral health.
AB - AIM: To compare the prevalence and levels of salivary Mutans Streptococci (MS) assessed with two commercial chair-side methods based on culture growth or monoclonal antibodies, respectively.MATERIAL AND METHODS: The study group consisted of a convenience sample of 89 adults, 23-72 years of age, referred to a maxillofacial hospital clinic with a caries history. Stimulated whole saliva samples were collected and the number of MS was assessed with the Dentocult-SM Strip Mutans (DSM) and the Saliva-Check Mutans (SCM). The outcome was compared with conventional anaerobic laboratory cultivation on selective MSB agar.RESULTS: The prevalence of ≥ 5 × 10(5) CFU was 21%, 62%, and 73% with the SCM, DSM and MSB-cultivation, respectively. The correlation between DSM and SCM tests was r = 0.49 (p < 0.05) but the agreement on the high levels was fair. In comparison with the MSB agar, DSM provided acceptable levels of sensitivity and specificity while the SCM displayed a poor sensitivity but a perfect specificity. Both chair-side methods were significantly related to the prevalence of active root caries lesions (p < 0.05).CONCLUSIONS: The two chair-side salivary tests were significantly correlated and due their high specificity, they may be sensitive enough for screening purposes and for patient-centered promotion of oral health.
U2 - 10.4172/2247-2452.1000630
DO - 10.4172/2247-2452.1000630
M3 - Journal article
C2 - 25284515
VL - 13
SP - 580
EP - 583
JO - Oral health and dental management
JF - Oral health and dental management
SN - 2247-2452
IS - 3
M1 - 630
ER -
ID: 137425138