Effects of TNF-α blocking on experimental periodontitis and type 2 diabetes in obese diabetic Zucker rats
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Effects of TNF-α blocking on experimental periodontitis and type 2 diabetes in obese diabetic Zucker rats. / Grauballe, Morten Bay; Østergaard, Jakob Appel; Schou, Søren; Flyvbjerg, Allan; Holmstrup, Palle.
In: Journal of Clinical Periodontology, Vol. 42, No. 9, 09.2015, p. 807-16.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Effects of TNF-α blocking on experimental periodontitis and type 2 diabetes in obese diabetic Zucker rats
AU - Grauballe, Morten Bay
AU - Østergaard, Jakob Appel
AU - Schou, Søren
AU - Flyvbjerg, Allan
AU - Holmstrup, Palle
N1 - © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
PY - 2015/9
Y1 - 2015/9
N2 - OBJECTIVE: Tumour necrosis factor α (TNF-α) is considered a key signalling modulator in the pathogenesis of both periodontitis (PD) and type 2 diabetes mellitus (DM2). This study aims at elucidating the effect of TNF-α blocking on the interplay between PD and DM2.METHODS: Obese diabetic Zucker rats and their lean littermates were divided into five treatment groups with or without periodontitis. Anti-TNF-α treatment was provided with Etanercept injections. Diabetic state was evaluated by oral glucose tolerance test, the homeostatic model assessment, free fatty acids and blood glucose. Systemic inflammation was assessed by measurement of interleukin (IL)-1β, IL-6 and TNF-α in plasma. Kidney complications were evaluated by real-time rtPCR, creatinine clearance rate, urinary albumin excretion and increase in weight. PD was evaluated by registration of alveolar bone level.RESULTS: After 4 weeks the diabetic state was modified by Etanercept treatment with lower insulin levels and lower homeostatic model assessment. Furthermore, while kidney complications were reduced by Etanercept treatment, PD had no effect. PD was influenced by diabetic state, but the impact was attenuated by Etanercept treatment.CONCLUSION: In this study anti-TNF-α treatment improved glucose tolerance and compensated for the increased periodontal disease in obese diabetic Zucker. PD did not influence diabetic parameters assessed including complications of the rats kidneys.
AB - OBJECTIVE: Tumour necrosis factor α (TNF-α) is considered a key signalling modulator in the pathogenesis of both periodontitis (PD) and type 2 diabetes mellitus (DM2). This study aims at elucidating the effect of TNF-α blocking on the interplay between PD and DM2.METHODS: Obese diabetic Zucker rats and their lean littermates were divided into five treatment groups with or without periodontitis. Anti-TNF-α treatment was provided with Etanercept injections. Diabetic state was evaluated by oral glucose tolerance test, the homeostatic model assessment, free fatty acids and blood glucose. Systemic inflammation was assessed by measurement of interleukin (IL)-1β, IL-6 and TNF-α in plasma. Kidney complications were evaluated by real-time rtPCR, creatinine clearance rate, urinary albumin excretion and increase in weight. PD was evaluated by registration of alveolar bone level.RESULTS: After 4 weeks the diabetic state was modified by Etanercept treatment with lower insulin levels and lower homeostatic model assessment. Furthermore, while kidney complications were reduced by Etanercept treatment, PD had no effect. PD was influenced by diabetic state, but the impact was attenuated by Etanercept treatment.CONCLUSION: In this study anti-TNF-α treatment improved glucose tolerance and compensated for the increased periodontal disease in obese diabetic Zucker. PD did not influence diabetic parameters assessed including complications of the rats kidneys.
U2 - 10.1111/jcpe.12442
DO - 10.1111/jcpe.12442
M3 - Journal article
C2 - 26257165
VL - 42
SP - 807
EP - 816
JO - Journal of Clinical Periodontology
JF - Journal of Clinical Periodontology
SN - 0303-6979
IS - 9
ER -
ID: 156560110