Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model. / Dabelsteen, Sally; Pallesen, Emil M. H.; Marinova, Irina N.; Nielsen, Mathias I.; Adamopoulou, Maria; Rømer, Troels B.; Levann, Asha; Andersen, Mikkel M.; Ye, Zilu; Thein, David; Bennett, Eric P; Büll, Christian; Moons, Sam J.; Boltje, Thomas; Clausen, Henrik; Vakhrushev, Sergey Y.; Bagdonaite, Ieva; Wandall, Hans H.

In: Developmental Cell, Vol. 54, No. 5, 2020, p. 669-684.e7.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Dabelsteen, S, Pallesen, EMH, Marinova, IN, Nielsen, MI, Adamopoulou, M, Rømer, TB, Levann, A, Andersen, MM, Ye, Z, Thein, D, Bennett, EP, Büll, C, Moons, SJ, Boltje, T, Clausen, H, Vakhrushev, SY, Bagdonaite, I & Wandall, HH 2020, 'Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model', Developmental Cell, vol. 54, no. 5, pp. 669-684.e7. https://doi.org/10.1016/j.devcel.2020.06.039

APA

Dabelsteen, S., Pallesen, E. M. H., Marinova, I. N., Nielsen, M. I., Adamopoulou, M., Rømer, T. B., Levann, A., Andersen, M. M., Ye, Z., Thein, D., Bennett, E. P., Büll, C., Moons, S. J., Boltje, T., Clausen, H., Vakhrushev, S. Y., Bagdonaite, I., & Wandall, H. H. (2020). Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model. Developmental Cell, 54(5), 669-684.e7. https://doi.org/10.1016/j.devcel.2020.06.039

Vancouver

Dabelsteen S, Pallesen EMH, Marinova IN, Nielsen MI, Adamopoulou M, Rømer TB et al. Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model. Developmental Cell. 2020;54(5):669-684.e7. https://doi.org/10.1016/j.devcel.2020.06.039

Author

Dabelsteen, Sally ; Pallesen, Emil M. H. ; Marinova, Irina N. ; Nielsen, Mathias I. ; Adamopoulou, Maria ; Rømer, Troels B. ; Levann, Asha ; Andersen, Mikkel M. ; Ye, Zilu ; Thein, David ; Bennett, Eric P ; Büll, Christian ; Moons, Sam J. ; Boltje, Thomas ; Clausen, Henrik ; Vakhrushev, Sergey Y. ; Bagdonaite, Ieva ; Wandall, Hans H. / Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model. In: Developmental Cell. 2020 ; Vol. 54, No. 5. pp. 669-684.e7.

Bibtex

@article{15f77bb05a864ea1a6c7d8584664b083,
title = "Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model",
abstract = "The glycome undergoes characteristic changes during histogenesis and organogenesis, but our understanding of the importance of select glycan structures for tissue formation and homeostasis is incomplete. Here, we present a human organotypic platform that allows genetic dissection of cellular glycosylation capacities and systematic interrogation of the roles of distinct glycan types in tissue formation. We used CRISPR-Cas9 gene targeting to generate a library of 3D organotypic skin tissues that selectively differ in their capacity to produce glycan structures on the main types of N- and O-linked glycoproteins and glycolipids. This tissue library revealed distinct changes in skin formation associated with a loss of features for all tested glycoconjugates. The organotypic skin model provides phenotypic cues for the distinct functions of glycoconjugates and serves as a unique resource for further genetic dissection and identification of the specific structural features involved. The strategy is also applicable to other organotypic tissue models.",
author = "Sally Dabelsteen and Pallesen, {Emil M. H.} and Marinova, {Irina N.} and Nielsen, {Mathias I.} and Maria Adamopoulou and R{\o}mer, {Troels B.} and Asha Levann and Andersen, {Mikkel M.} and Zilu Ye and David Thein and Bennett, {Eric P} and Christian B{\"u}ll and Moons, {Sam J.} and Thomas Boltje and Henrik Clausen and Vakhrushev, {Sergey Y.} and Ieva Bagdonaite and Wandall, {Hans H.}",
year = "2020",
doi = "10.1016/j.devcel.2020.06.039",
language = "English",
volume = "54",
pages = "669--684.e7",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "5",

}

RIS

TY - JOUR

T1 - Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model

AU - Dabelsteen, Sally

AU - Pallesen, Emil M. H.

AU - Marinova, Irina N.

AU - Nielsen, Mathias I.

AU - Adamopoulou, Maria

AU - Rømer, Troels B.

AU - Levann, Asha

AU - Andersen, Mikkel M.

AU - Ye, Zilu

AU - Thein, David

AU - Bennett, Eric P

AU - Büll, Christian

AU - Moons, Sam J.

AU - Boltje, Thomas

AU - Clausen, Henrik

AU - Vakhrushev, Sergey Y.

AU - Bagdonaite, Ieva

AU - Wandall, Hans H.

PY - 2020

Y1 - 2020

N2 - The glycome undergoes characteristic changes during histogenesis and organogenesis, but our understanding of the importance of select glycan structures for tissue formation and homeostasis is incomplete. Here, we present a human organotypic platform that allows genetic dissection of cellular glycosylation capacities and systematic interrogation of the roles of distinct glycan types in tissue formation. We used CRISPR-Cas9 gene targeting to generate a library of 3D organotypic skin tissues that selectively differ in their capacity to produce glycan structures on the main types of N- and O-linked glycoproteins and glycolipids. This tissue library revealed distinct changes in skin formation associated with a loss of features for all tested glycoconjugates. The organotypic skin model provides phenotypic cues for the distinct functions of glycoconjugates and serves as a unique resource for further genetic dissection and identification of the specific structural features involved. The strategy is also applicable to other organotypic tissue models.

AB - The glycome undergoes characteristic changes during histogenesis and organogenesis, but our understanding of the importance of select glycan structures for tissue formation and homeostasis is incomplete. Here, we present a human organotypic platform that allows genetic dissection of cellular glycosylation capacities and systematic interrogation of the roles of distinct glycan types in tissue formation. We used CRISPR-Cas9 gene targeting to generate a library of 3D organotypic skin tissues that selectively differ in their capacity to produce glycan structures on the main types of N- and O-linked glycoproteins and glycolipids. This tissue library revealed distinct changes in skin formation associated with a loss of features for all tested glycoconjugates. The organotypic skin model provides phenotypic cues for the distinct functions of glycoconjugates and serves as a unique resource for further genetic dissection and identification of the specific structural features involved. The strategy is also applicable to other organotypic tissue models.

U2 - 10.1016/j.devcel.2020.06.039

DO - 10.1016/j.devcel.2020.06.039

M3 - Journal article

C2 - 32710848

VL - 54

SP - 669-684.e7

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 5

ER -

ID: 245659289