Experimental Pseudomonas aeruginosa mediated rhino sinusitis in mink
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Experimental Pseudomonas aeruginosa mediated rhino sinusitis in mink. / Kirkeby, S; Hammer, A S; Høiby, N; Salomonsen, C.M.
In: International Journal of Pediatric Otorhinolaryngology, Vol. 96, 05.2017, p. 156-163.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Experimental Pseudomonas aeruginosa mediated rhino sinusitis in mink
AU - Kirkeby, S
AU - Hammer, A S
AU - Høiby, N
AU - Salomonsen, C.M.
N1 - Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.
PY - 2017/5
Y1 - 2017/5
N2 - OBJECTIVES: The nasal and sinus cavities in children may serve as reservoirs for microorganisms that cause recurrent and chronic lung infections. This study evaluates whether the mink can be used as an animal model for studying Pseudomonas aeruginosa mediated rhino-sinusitis since there is no suitable traditional animal model for this disease.METHODS: Nasal tissue samples from infected and control mink were fixed in formalin, demineralized, and embedded in paraffin. A histological examination of sections from the infected animals revealed disintegration of the respiratory epithelium lining the nasal turbinates and swelling and edema of the submucosa. The expression of mucins and sialylated glycans was examined using immunohistochemistry.RESULTS: MUC1, MUC2 and MUC5AC were upregulated in the inoculated animals as a much stronger staining was present in the respiratory epithelium in the infected animals compared to the controls. The goblet cells in the nasal epithelium from the infected mink showed high affinity to the Maackia amurensis lectin and anti-asialo GM1 indicating a high concentration of α2-3 sialic acid respectively βGalNAc1-4Galβ containing glycans in these mucin producing cells. The nasal cavity in the infected mink shows features of carbohydrate expression comparable to what has been described in the respiratory system after Pseudomonas aeruginosa infection in humans.CONCLUSION: It is suggested that the mink is suitable for studying Pseudomonas aeruginosa mediated rhino-sinusitis.
AB - OBJECTIVES: The nasal and sinus cavities in children may serve as reservoirs for microorganisms that cause recurrent and chronic lung infections. This study evaluates whether the mink can be used as an animal model for studying Pseudomonas aeruginosa mediated rhino-sinusitis since there is no suitable traditional animal model for this disease.METHODS: Nasal tissue samples from infected and control mink were fixed in formalin, demineralized, and embedded in paraffin. A histological examination of sections from the infected animals revealed disintegration of the respiratory epithelium lining the nasal turbinates and swelling and edema of the submucosa. The expression of mucins and sialylated glycans was examined using immunohistochemistry.RESULTS: MUC1, MUC2 and MUC5AC were upregulated in the inoculated animals as a much stronger staining was present in the respiratory epithelium in the infected animals compared to the controls. The goblet cells in the nasal epithelium from the infected mink showed high affinity to the Maackia amurensis lectin and anti-asialo GM1 indicating a high concentration of α2-3 sialic acid respectively βGalNAc1-4Galβ containing glycans in these mucin producing cells. The nasal cavity in the infected mink shows features of carbohydrate expression comparable to what has been described in the respiratory system after Pseudomonas aeruginosa infection in humans.CONCLUSION: It is suggested that the mink is suitable for studying Pseudomonas aeruginosa mediated rhino-sinusitis.
KW - Animals
KW - Disease Models, Animal
KW - Immunohistochemistry
KW - Mink
KW - Mucins
KW - Nasal Mucosa
KW - Pseudomonas Infections
KW - Pseudomonas aeruginosa
KW - Rhinitis
KW - Sinusitis
KW - Journal Article
U2 - 10.1016/j.ijporl.2016.12.037
DO - 10.1016/j.ijporl.2016.12.037
M3 - Journal article
C2 - 28302328
VL - 96
SP - 156
EP - 163
JO - International Journal of Pediatric Otorhinolaryngology Extra
JF - International Journal of Pediatric Otorhinolaryngology Extra
SN - 1871-4048
ER -
ID: 178523956