Glycoengineering of Human Cell Lines Using Zinc Finger Nuclease Gene Targeting: SimpleCells with Homogeneous GalNAc O-glycosylation Allow Isolation of the O-glycoproteome by One-Step Lectin Affinity Chromatography

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Communication

Standard

Glycoengineering of Human Cell Lines Using Zinc Finger Nuclease Gene Targeting : SimpleCells with Homogeneous GalNAc O-glycosylation Allow Isolation of the O-glycoproteome by One-Step Lectin Affinity Chromatography. / Steentoft, Catharina; Bennett, Eric Paul; Clausen, Henrik.

Glycosyltransferases: Methods and Protocols. ed. / Inka Brockhausen . Vol. 1022 Humana Press, 2013. p. 387-402 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Communication

Harvard

Steentoft, C, Bennett, EP & Clausen, H 2013, Glycoengineering of Human Cell Lines Using Zinc Finger Nuclease Gene Targeting: SimpleCells with Homogeneous GalNAc O-glycosylation Allow Isolation of the O-glycoproteome by One-Step Lectin Affinity Chromatography. in I Brockhausen (ed.), Glycosyltransferases: Methods and Protocols. vol. 1022, Humana Press, Methods in molecular biology (Clifton, N.J.), pp. 387-402. https://doi.org/10.1007/978-1-62703-465-4_29

APA

Steentoft, C., Bennett, E. P., & Clausen, H. (2013). Glycoengineering of Human Cell Lines Using Zinc Finger Nuclease Gene Targeting: SimpleCells with Homogeneous GalNAc O-glycosylation Allow Isolation of the O-glycoproteome by One-Step Lectin Affinity Chromatography. In I. Brockhausen (Ed.), Glycosyltransferases: Methods and Protocols (Vol. 1022, pp. 387-402). Humana Press. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-62703-465-4_29

Vancouver

Steentoft C, Bennett EP, Clausen H. Glycoengineering of Human Cell Lines Using Zinc Finger Nuclease Gene Targeting: SimpleCells with Homogeneous GalNAc O-glycosylation Allow Isolation of the O-glycoproteome by One-Step Lectin Affinity Chromatography. In Brockhausen I, editor, Glycosyltransferases: Methods and Protocols. Vol. 1022. Humana Press. 2013. p. 387-402. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-62703-465-4_29

Author

Steentoft, Catharina ; Bennett, Eric Paul ; Clausen, Henrik. / Glycoengineering of Human Cell Lines Using Zinc Finger Nuclease Gene Targeting : SimpleCells with Homogeneous GalNAc O-glycosylation Allow Isolation of the O-glycoproteome by One-Step Lectin Affinity Chromatography. Glycosyltransferases: Methods and Protocols. editor / Inka Brockhausen . Vol. 1022 Humana Press, 2013. pp. 387-402 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{740caafd793d4629af2cb5007060709b,
title = "Glycoengineering of Human Cell Lines Using Zinc Finger Nuclease Gene Targeting: SimpleCells with Homogeneous GalNAc O-glycosylation Allow Isolation of the O-glycoproteome by One-Step Lectin Affinity Chromatography",
abstract = "Lectin affinity chromatography is a powerful technique for isolation of glycoproteins carrying a specific glycan structure of interest. However, the enormous diversity of glycans present on the cell surface, as well as on individual proteins, makes it difficult to isolate an entire glycoproteome with one or even a series of lectins. Here we present a technique to generate cell lines with homogenous truncated O-glycans using zinc finger nuclease gene targeting. Because of their simplified O-glycoproteome, the cells have been named SimpleCells. Glycoproteins from SimpleCells can be isolated in a single purification step by lectin chromatography performed on a long lectin column. This protocol describes Zinc finger nuclease gene targeting of human cells to simplify the glycoproteome, as well as lectin chromatography and isolation of glycopeptides from total cell lysates of SimpleCells.",
author = "Catharina Steentoft and Bennett, {Eric Paul} and Henrik Clausen",
year = "2013",
doi = "10.1007/978-1-62703-465-4_29",
language = "English",
isbn = "978-1-62703-464-7",
volume = "1022",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "387--402",
editor = "{Brockhausen }, Inka",
booktitle = "Glycosyltransferases",
address = "United States",

}

RIS

TY - CHAP

T1 - Glycoengineering of Human Cell Lines Using Zinc Finger Nuclease Gene Targeting

T2 - SimpleCells with Homogeneous GalNAc O-glycosylation Allow Isolation of the O-glycoproteome by One-Step Lectin Affinity Chromatography

AU - Steentoft, Catharina

AU - Bennett, Eric Paul

AU - Clausen, Henrik

PY - 2013

Y1 - 2013

N2 - Lectin affinity chromatography is a powerful technique for isolation of glycoproteins carrying a specific glycan structure of interest. However, the enormous diversity of glycans present on the cell surface, as well as on individual proteins, makes it difficult to isolate an entire glycoproteome with one or even a series of lectins. Here we present a technique to generate cell lines with homogenous truncated O-glycans using zinc finger nuclease gene targeting. Because of their simplified O-glycoproteome, the cells have been named SimpleCells. Glycoproteins from SimpleCells can be isolated in a single purification step by lectin chromatography performed on a long lectin column. This protocol describes Zinc finger nuclease gene targeting of human cells to simplify the glycoproteome, as well as lectin chromatography and isolation of glycopeptides from total cell lysates of SimpleCells.

AB - Lectin affinity chromatography is a powerful technique for isolation of glycoproteins carrying a specific glycan structure of interest. However, the enormous diversity of glycans present on the cell surface, as well as on individual proteins, makes it difficult to isolate an entire glycoproteome with one or even a series of lectins. Here we present a technique to generate cell lines with homogenous truncated O-glycans using zinc finger nuclease gene targeting. Because of their simplified O-glycoproteome, the cells have been named SimpleCells. Glycoproteins from SimpleCells can be isolated in a single purification step by lectin chromatography performed on a long lectin column. This protocol describes Zinc finger nuclease gene targeting of human cells to simplify the glycoproteome, as well as lectin chromatography and isolation of glycopeptides from total cell lysates of SimpleCells.

U2 - 10.1007/978-1-62703-465-4_29

DO - 10.1007/978-1-62703-465-4_29

M3 - Book chapter

C2 - 23765677

SN - 978-1-62703-464-7

VL - 1022

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 387

EP - 402

BT - Glycosyltransferases

A2 - Brockhausen , Inka

PB - Humana Press

ER -

ID: 47686302