Influences of tumor stroma on the malignant phenotype.

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Influences of tumor stroma on the malignant phenotype. / Nielsen, Jørgen Dau; Moeslund, Mette; Wandall, Hans H; Dabelsteen, Sally.

In: Journal of Oral Pathology & Medicine, Vol. 37, No. 7, 2008, p. 412-6.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nielsen, JD, Moeslund, M, Wandall, HH & Dabelsteen, S 2008, 'Influences of tumor stroma on the malignant phenotype.', Journal of Oral Pathology & Medicine, vol. 37, no. 7, pp. 412-6. https://doi.org/10.1111/j.1600-0714.2008.00655.x

APA

Nielsen, J. D., Moeslund, M., Wandall, H. H., & Dabelsteen, S. (2008). Influences of tumor stroma on the malignant phenotype. Journal of Oral Pathology & Medicine, 37(7), 412-6. https://doi.org/10.1111/j.1600-0714.2008.00655.x

Vancouver

Nielsen JD, Moeslund M, Wandall HH, Dabelsteen S. Influences of tumor stroma on the malignant phenotype. Journal of Oral Pathology & Medicine. 2008;37(7):412-6. https://doi.org/10.1111/j.1600-0714.2008.00655.x

Author

Nielsen, Jørgen Dau ; Moeslund, Mette ; Wandall, Hans H ; Dabelsteen, Sally. / Influences of tumor stroma on the malignant phenotype. In: Journal of Oral Pathology & Medicine. 2008 ; Vol. 37, No. 7. pp. 412-6.

Bibtex

@article{7d6e7090849b11dd81b0000ea68e967b,
title = "Influences of tumor stroma on the malignant phenotype.",
abstract = "BACKGROUND: The microenvironment makes a significant contribution to the progression of oral carcinomas. Many different stromal events, such as vascularization, fibroblast activation, myofibroblast differentiation, and the presence of specific stromal proteins, such as proteolytic enzymes, fibronectin and laminin 5 are all characteristics of the tumor stroma. Less is, however, known of the significance of the biophysical properties of the tumor stroma. The purpose of the present study was to investigate how cellular and mechanical properties of the three-dimensional collagen matrix may influence cell proliferation and invasion of oral carcinoma cell lines. METHOD: Oral cancer cells were cultured in an organotypic culture system on different collagen concentrations, and invasion was measured. Furthermore, the presence of cancer associated proteins such as glycosylated oncofetal fibronectin and laminin 5 was investigated. RESULTS: We found that expression of glycosylated oncofetal fibronectin was increased in the invasive phenotype of oral carcinoma cell lines. Furthermore we demonstrated that certain concentrations of collagen in the connective tissue equivalent, appears to stimulate invasiveness of oral carcinoma cells.",
author = "Nielsen, {J{\o}rgen Dau} and Mette Moeslund and Wandall, {Hans H} and Sally Dabelsteen",
year = "2008",
doi = "10.1111/j.1600-0714.2008.00655.x",
language = "English",
volume = "37",
pages = "412--6",
journal = "Journal of Oral Pathology and Medicine",
issn = "0904-2512",
publisher = "Wiley-Blackwell",
number = "7",

}

RIS

TY - JOUR

T1 - Influences of tumor stroma on the malignant phenotype.

AU - Nielsen, Jørgen Dau

AU - Moeslund, Mette

AU - Wandall, Hans H

AU - Dabelsteen, Sally

PY - 2008

Y1 - 2008

N2 - BACKGROUND: The microenvironment makes a significant contribution to the progression of oral carcinomas. Many different stromal events, such as vascularization, fibroblast activation, myofibroblast differentiation, and the presence of specific stromal proteins, such as proteolytic enzymes, fibronectin and laminin 5 are all characteristics of the tumor stroma. Less is, however, known of the significance of the biophysical properties of the tumor stroma. The purpose of the present study was to investigate how cellular and mechanical properties of the three-dimensional collagen matrix may influence cell proliferation and invasion of oral carcinoma cell lines. METHOD: Oral cancer cells were cultured in an organotypic culture system on different collagen concentrations, and invasion was measured. Furthermore, the presence of cancer associated proteins such as glycosylated oncofetal fibronectin and laminin 5 was investigated. RESULTS: We found that expression of glycosylated oncofetal fibronectin was increased in the invasive phenotype of oral carcinoma cell lines. Furthermore we demonstrated that certain concentrations of collagen in the connective tissue equivalent, appears to stimulate invasiveness of oral carcinoma cells.

AB - BACKGROUND: The microenvironment makes a significant contribution to the progression of oral carcinomas. Many different stromal events, such as vascularization, fibroblast activation, myofibroblast differentiation, and the presence of specific stromal proteins, such as proteolytic enzymes, fibronectin and laminin 5 are all characteristics of the tumor stroma. Less is, however, known of the significance of the biophysical properties of the tumor stroma. The purpose of the present study was to investigate how cellular and mechanical properties of the three-dimensional collagen matrix may influence cell proliferation and invasion of oral carcinoma cell lines. METHOD: Oral cancer cells were cultured in an organotypic culture system on different collagen concentrations, and invasion was measured. Furthermore, the presence of cancer associated proteins such as glycosylated oncofetal fibronectin and laminin 5 was investigated. RESULTS: We found that expression of glycosylated oncofetal fibronectin was increased in the invasive phenotype of oral carcinoma cell lines. Furthermore we demonstrated that certain concentrations of collagen in the connective tissue equivalent, appears to stimulate invasiveness of oral carcinoma cells.

U2 - 10.1111/j.1600-0714.2008.00655.x

DO - 10.1111/j.1600-0714.2008.00655.x

M3 - Journal article

C2 - 18331288

VL - 37

SP - 412

EP - 416

JO - Journal of Oral Pathology and Medicine

JF - Journal of Oral Pathology and Medicine

SN - 0904-2512

IS - 7

ER -

ID: 6091899