The influence of temperature on the distribution and intensity of the reaction product in rat muscle fibers obtained with the histochemical method for myosin ATPase
Research output: Contribution to journal › Journal article › Research › peer-review
The influence of temperature in the incubation medium on the localization and intensity of myosin ATPase was investigated in striated muscles from the rat using a conventional histochemical technique. It was found that the enzyme reaction was temperature-dependent since the activity in some fibers was raised and in others was depressed by alteration of the incubation temperature. There was no obvious correlation between the temperature sensitivity of ATPase in the muscle fibers and their activity for succinic dehydrogenase. It is proposed that the histochemical method for myosin ATPase can be used for demonstration of isoenzymes in striated muscle fibers.
Original language | English |
---|---|
Journal | Journal of Neuroscience Methods |
Volume | 26 |
Issue number | 3 |
Pages (from-to) | 189-94 |
Number of pages | 5 |
ISSN | 0165-0270 |
Publication status | Published - 1989 |
Bibliographical note
Keywords: Animals; Histocytochemistry; Male; Masseter Muscle; Muscles; Myosins; Rats; Rats, Inbred Strains; Temperature
ID: 10153978